SAFB2
Reactivity: Human
IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunoprecipitation. Immunoflourescence. Western blot. Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 - -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.Synonyms: KIAA0138, SAF-B2, Scaffold attachment factor B2