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Recombinant Calponin antibody

This anti-Calponin antibody is a Rabbit Monoclonal antibody detecting Calponin in IHC (p), IF and FACS. Suitable for Human.
Catalog No. ABIN3023710

Quick Overview for Recombinant Calponin antibody (ABIN3023710)

Target

Calponin

Antibody Type

Recombinant Antibody

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This Calponin antibody is un-conjugated

Application

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone

RMCN1-1
  • Purification

    Protein A affinity chromatography

    Immunogen

    Recombinant protein was used as the immunogen for the Calponin antibody.

    Isotype

    IgG kappa
  • Application Notes

    Optimal dilution of the Calponin antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min\. FACS: 0.5-1 μg/10^6 cells,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Preservative

    Azide free

    Storage

    4 °C,-20 °C

    Storage Comment

    Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    Calponin

    Background

    Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.
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