Calponin antibody

Catalog No. ABIN3025295

This Mouse Monoclonal antibody specifically detects Calponin in IHC (p), WB and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for Calponin antibody (ABIN3025295)

Target: Calponin

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calponin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunofluorescence (IF)

Grade: Carrier-free

Clone: CNN1-832

Product Details anti-Calponin Antibody

Purpose: Anti-Calponin Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the anti-Calponin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the anti-Calponin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Calponin

Target: Calponin

Background: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

UniProt: P51911