Recombinant Calponin antibody

Catalog No. ABIN4949947

Product Details anti-Calponin Antibody

Target: Calponin

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Calponin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Purity: Protein A affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for the Calponin antibody.

Clone: RMCN1-1

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the Calponin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min\. FACS: 0.5-1 μg/10^6 cells,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Calponin

Target: Calponin

Abstract: Calponin Products

Synonyms: CalPoNin antibody, cpn-1 antibody, cpn-2 antibody

Background: Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.