CPS1 antibody

Catalog No. ABIN3023811

The Mouse Monoclonal anti-CPS1 antibody has been validated for IF and IHC (p). It is suitable to detect CPS1 in samples from Human and Dog.

Quick Overview for CPS1 antibody (ABIN3023811)

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CPS1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CPS1-1022

Product Details anti-CPS1 Antibody

Characteristics: This mAb recognizes a protein of 165 kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the CPS1 antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the CPS1 antibody should be determined by the researcher.

1.Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CPS1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CPS1

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Alternative Name: CPS1

Background: This mAb recognizes a protein of 165  kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

Pathways: Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process