Keratin 10 antibody

Catalog No. ABIN3023828

This Mouse Monoclonal antibody specifically detects Keratin 10 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Keratin 10 antibody (ABIN3023828)

Target: Keratin 10 (KRT10)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KRT10-844

Product Details anti-Keratin 10 Antibody

Purpose: Keratin 10 Antibody / KRT10

Characteristics: This mAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Purification: Protein G affinity chromatography

Immunogen: Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Keratin 10 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Keratin 10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Keratin 10

Background: This mAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

UniProt: P13645