beta Catenin antibody

Catalog No. ABIN3024225

The Rabbit Polyclonal anti-beta Catenin antibody has been validated for WB, FACS, IF and IHC (p). It is suitable to detect beta Catenin in samples from Human and Mouse.

Quick Overview for beta Catenin antibody (ABIN3024225)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-beta Catenin Antibody

Purification: Protein A affinity chromatography

Immunogen: A synthetic peptide from the middle of beta-Catenin / p120 protein was used as the immunogen.

Isotype: IgG

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the beta-Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: beta-Catenin

Background: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, it is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution throughout the cytoplasm results in a diffuse cytoplasmic pattern. Staining with beta-catenin antibody and E-cadherin antibody helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Gene ID: 1499

Pathways: Peptide Hormone Metabolism