Melanoma gp100 antibody

Catalog No. ABIN3024239

This Mouse Monoclonal antibody specifically detects Melanoma gp100 in IF, FACS and IHC (p). It exhibits reactivity toward Human and Horse.

Quick Overview for Melanoma gp100 antibody (ABIN3024239)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM286

Product Details anti-Melanoma gp100 Antibody

No Cross-Reactivity: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: The optimal dilution of the anti-gp100 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-gp100 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: Gp100

Background: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.