Calponin antibody

Catalog No. ABIN3024264

The Mouse Monoclonal anti-Calponin antibody is suitable to detect Calponin in samples from Human and Rat. It has been validated for IHC (p) and IF.

Quick Overview for Calponin antibody (ABIN3024264)

Target: Calponin

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calponin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: CLPN5-1

Product Details anti-Calponin Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the Calponin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Calponin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Calponin

Target: Calponin

Background: Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.