NGFR antibody

Catalog No. ABIN3024324

The Mouse Monoclonal anti-NGFR antibody has been validated for FACS, IF and IHC (p). It is suitable to detect NGFR in samples from Human and Rat.

Quick Overview for NGFR antibody (ABIN3024324)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CDLA271

Product Details anti-NGFR Antibody

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the CD271 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the CD271 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD271 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: CD271

Background: CD271/NGFR pays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt]

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding