CD45RB antibody

Catalog No. ABIN3024394

This anti-CD45RB antibody is a Mouse Monoclonal antibody detecting CD45RB in FACS, IF and IHC (p). Suitable for Human.

Quick Overview for CD45RB antibody (ABIN3024394)

Target: CD45RB

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RB antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DF-B1

Product Details anti-CD45RB Antibody

Purification: Protein G affinity chromatography

Immunogen: Extract from the hepatoma cell line L428 was used as immunogen for this CD45RB antibody.

Isotype: IgG3 kappa

Application Details

Application Notes:

Due to variation in protocol and secondary antibody used, the CD45RB antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells,Immunofluorescence: 0.5-1 μg/mL,Immunohistology (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: The CD45RB antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD45RB

Target: CD45RB

Alternative Name: CD45RB

Background: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage.