GPR68 antibody (N-Term)

Catalog No. ABIN302446

Product Details anti-GPR68 Antibody

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR68 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects OGR1 / GPR68 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse (others see 'Immunogen Remarks')Species reactivity (tested):Human

Purification: Immunoaffinity chromatography

Immunogen: Synthetic 17 amino acid peptide from N-terminal domain of human GPR68 / OGR1

Application Details

Application Notes: Immunohistochemistry on paraffin sections (20 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -80 °C for longer.

Target Details for GPR68

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Alternative Name: GPR68 / OGR1 (GPR68 Products)

Synonyms: GPR68 antibody, GPR12A antibody, OGR1 antibody, BB131428 antibody, Ogr1 antibody, G protein-coupled receptor 68 antibody, GPR68 antibody, LOC100348269 antibody, Gpr68 antibody

Background: OGR1, a Lysophospholipid/Lysosphingolipid Receptor, was isolated by degenerate PCR from an ovarian cell line cDNA library. It is not expressed in normal ovary. OGR1 binds sphingosylphosporylcholine, a lipid molecule involved in cell growth and survival. Recently, OGR1 has been shown to act as a proton-sensing receptor stimulating inositol phosphate formation (Ludwig et al. 2003).Synonyms: G-protein coupled receptor 68, GPR12A, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Gene ID: 8111

NCBI Accession: NP_001171147

UniProt: Q15743