Cytokeratin 8/18 antibody

Catalog No. ABIN3024678

The Mouse Monoclonal anti-Cytokeratin 8/18 antibody is suitable to detect Cytokeratin 8/18 in samples from Human. It has been validated for IF, WB, FACS and IHC (p).

Quick Overview for Cytokeratin 8/18 antibody (ABIN3024678)

Target: Cytokeratin 8/18

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 8/18 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: C-51

Product Details anti-Cytokeratin 8/18 Antibody

Purpose: Cytokeratin 8/18 Antibody (azide and preservative free)

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Ferret, Rabbit, Donkey, Chicken, Xenopus laevis

Purification: Protein G affinity chromatography

Immunogen: A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8/18 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Cytokeratin 8/18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Cytokeratin 8/18

Target: Cytokeratin 8/18

Background: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

UniProt: P05787