EpCAM antibody

Catalog No. ABIN3024720

The Mouse Monoclonal anti-EpCAM antibody has been validated for WB, FACS, IF and IHC (p). It is suitable to detect EpCAM in samples from Mouse and Rat.

Quick Overview for EpCAM antibody (ABIN3024720)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: Epcam-1158

Product Details anti-EpCAM Antibody

No Cross-Reactivity: Human

Purity: Unpurified high titer supernatant

Immunogen: Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the rat EpCAM antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The epitopes of mAbs Epcam/1158 and < a href=../tds/rat-epcam-antibody-epcam-1159-v3118s>Epcam/1159 monoclonal antibodies are different.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 5-10 μL/million cells in 0.1ml,Immunofluorescence: 1:50-1:100,Western blot: 1:100-1:200,Immunohistochemistry (FFPE): 1:50-1:100 30 min at RT (1),Prediluted format: incubate for 30 min at RT (3)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Bioreactor concentrate with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Store the rat EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM

Background: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.