Elastase 3B antibody (AA 82-238)

Catalog No. ABIN3024740

This Mouse Monoclonal antibody specifically detects Elastase 3B in ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Elastase 3B antibody (AA 82-238) (ABIN3024740)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastase 3B antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ELTS3B-2

Product Details anti-Elastase 3B Antibody

Binding Specificity: AA 82-238

Purification: Protein G

Immunogen: A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

1. FFPE staining requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. ELISA: 2-4 μg/mL, order BSA free format for coating,IF: 1-2 μg/mL, IHC-P: 1-2 μg/mL (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Elastase 3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Alternative Name: Elastase 3B

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.