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Recombinant Elastase 3B antibody (AA 82-238)

ELA3B Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal rCELA3B-1811 unconjugated Recombinant Antibody
Catalog No. ABIN6939416
  • Target See all Elastase 3B (ELA3B) Antibodies
    Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
    Antibody Type
    Recombinant Antibody
    Binding Specificity
    • 53
    • 9
    • 7
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 82-238
    Reactivity
    • 80
    • 20
    • 19
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 54
    • 35
    Mouse
    Clonality
    • 58
    • 31
    Monoclonal
    Conjugate
    • 42
    • 15
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    This Elastase 3B antibody is un-conjugated
    Application
    • 61
    • 59
    • 44
    • 41
    • 39
    • 38
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificity
    This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)
    Clone
    RCELA3B-1811
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product ELA3B Primary Antibody
  • Application Notes

    Positive Control: Panc-28 or PANC1 cells. Human Pancreas.

    Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
    Alternative Name
    CELA3B (ELA3B Products)
    Synonyms
    CBPP antibody, E1 antibody, EL-1 antibody, ELA3B antibody, 0910001F22Rik antibody, 2310074F01Rik antibody, AI504000 antibody, Ela3 antibody, Ela3b antibody, ela3b antibody, MGC82867 antibody, ELA1 antibody, Cela3b antibody, CELA3B antibody, chymotrypsin like elastase family member 3B antibody, chymotrypsin-like elastase family, member 3B antibody, elastase 3B, pancreatic antibody, proproteinase E antibody, chymotrypsin like elastase family member 3B L homeolog antibody, chymotrypsin-like elastase family member 3B antibody, CELA3B antibody, Cela3b antibody, ela3b antibody, LOC478196 antibody, cela3b.L antibody, LOC100717936 antibody, LOC100071692 antibody
    Molecular Weight
    ~17kDa
    Gene ID
    23436
    UniProt
    P08861
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