Tyrosinase-Related Protein 1 antibody

Catalog No. ABIN3024976

This anti-Tyrosinase-Related Protein 1 antibody is a Mouse Monoclonal antibody detecting Tyrosinase-Related Protein 1 in IF, FACS and IHC (p). Suitable for Human and Mouse.

Quick Overview for Tyrosinase-Related Protein 1 antibody (ABIN3024976)

Target: Tyrosinase-Related Protein 1 (TYRP1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tyrosinase-Related Protein 1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM611

Product Details anti-Tyrosinase-Related Protein 1 Antibody

Characteristics: It reacts with a 75 kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the TRP1 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the TRP1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the TRP1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Alternative Name: TRP1

Background: It reacts with a 75  kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.