Tyrosinase-Related Protein 1 antibody

Catalog No. ABIN6940843

This Rabbit Polyclonal antibody specifically detects Tyrosinase-Related Protein 1 in IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for Tyrosinase-Related Protein 1 antibody (ABIN6940843)

Target: Tyrosinase-Related Protein 1 (TYRP1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosinase-Related Protein 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-Tyrosinase-Related Protein 1 Antibody

Purpose: Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker) Antibody

Specificity: It reacts with a 75 kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi.Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

Purification: 200ug/ml of Ab Purified by Protein A.

Immunogen: Recombinant human TYRP1 protein

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells. Melanoma.

Known Application: Immunohistochemistry (Formalin-fixed) (5-10 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Alternative Name: TYRP1

Background:

5, 6 dihydroxyindole 2 carboxylic acid oxidase, 6-dihydroxyindole-2-carboxylic acid oxidase, Associated with iris pigmentation, CAS2, Catalase B (CATB), DHICA oxidase, Glycoprotein75 (GP75), Melanoma antigen gp75, Tyrosinase-related protein 1 (TYRP1), TYRRP

SK-MEL-19, SK-MEL-23, SK-MEL-30, SK-MEL-37 cells. Melanoma.

Molecular Weight: 75kDa

Gene ID: 7306, 270279

UniProt: P17643