LSD1 antibody (N-Term)

Catalog No. ABIN3025191

The Rabbit Polyclonal anti-LSD1 antibody is suitable to detect LSD1 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for LSD1 antibody (N-Term) (ABIN3025191)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LSD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LSD1 Antibody

Binding Specificity: N-Term

Purification: Protein A affinity chromatography

Immunogen: A recombinant protein fragment from the N-terminal region of human LSD1 / KDM1 was used as the immunogen for this antibody.

Isotype: IgG

Application Details

Application Notes: Provided assay concentrations are suggestions only, LSD1 / KDM1 antibody titration may be required for optimal results.\. Western blot: 0.5-2 μg/mL, IHC (FFPE): 5 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the LSD1 / KDM1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Alternative Name: LSD1 / KDM1

Background: LSD1 (Lysine-specific demethylase 1), also called KDM1 (lysine (K)-specific demethylase 1A), is the first histone demethylase to be identified and regulates transcription via demethylation of lysines 4 and 9 of Histone H3. It negatively regulates pro-inflammatory cytokine expression by interacting with the genes promoter region. It also plays a role in tumor development where overexpression of LSD1 correlates with a poor prognosis. It is upregulated in cancers of the bladder, breast, lung, colon, and GI tract as well as neuroblastoma and cells of mesenchymal origin such as chondrosarcoma, osteosarcoma, rhabdomyosarcoma and Ewing's sarcoma (Bennani-Baiti et al, 2012).

Gene ID: 23028

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect