MITF antibody (N-Term)

Catalog No. ABIN3025230

The Mouse Monoclonal anti-MITF antibody has been validated for IF, FACS and IHC (p). It is suitable to detect MITF in samples from Human.

Quick Overview for MITF antibody (N-Term) (ABIN3025230)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM290

Product Details anti-MITF Antibody

Binding Specificity: N-Term

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: An N-terminus fragment of human Mi protein was used as the immunogen for the anti-MITF antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the anti-MITF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-MITF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, -B, -C, -H, and -M, which differ in the amino-terminal domain and in their expression patterns. The M isoform is restricted to the melanocyte cell lineage.

Pathways: Chromatin Binding