Recombinant Nkx2-2 antibody

Catalog No. ABIN3025346

This Rabbit Monoclonal antibody specifically detects Nkx2-2 in IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant Nkx2-2 antibody (ABIN3025346)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RMNK2-1

Product Details anti-Nkx2-2 Antibody

Purification: Protein A affinity chromatography

Immunogen: A human partial recombinant protein was used as the immunogen for this rabbit monoclonal NKX2.2 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the NKX2.2 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2

Background: NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity). [UniProt]

Gene ID: 4821

Pathways: Dopaminergic Neurogenesis