Prosaposin antibody

Catalog No. ABIN3025433

This Mouse Monoclonal antibody specifically detects Prosaposin in IF, IHC (p) and FACS. It exhibits reactivity toward Human.

Quick Overview for Prosaposin antibody (ABIN3025433)

Target: Prosaposin (PSAP)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prosaposin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: PSPN1-1

Product Details anti-Prosaposin Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for this PSAP antibody.

Isotype: IgG1

Application Details

Application Notes: Titering of the PSAP antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml, Immunofluorescence: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: The PSAP antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Prosaposin

Target: Prosaposin (PSAP)

Alternative Name: PSAP / Prostate Specific Acid Phosphatase

Background: Prostate specific acid phosphatase (PSAP) is an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.

Gene ID: 55

Pathways: Positive Regulation of Endopeptidase Activity