Human Papilloma Virus 16 Capsid Protein (HPV-16 L1) antibody

Catalog No. ABIN3025478

This Mouse Monoclonal antibody specifically detects Human Papilloma Virus 16 Capsid Protein in IHC (p), WB, IP and IF. It exhibits reactivity toward Human Papillomavirus 16 (HPV-16).

Quick Overview for Human Papilloma Virus 16 Capsid Protein (HPV-16 L1) antibody (ABIN3025478)

Target: Human Papilloma Virus 16 Capsid Protein (HPV-16 L1)

Reactivity: Human Papillomavirus 16 (HPV-16)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: HPV16L1-1058

Product Details

Characteristics: Reacts with a protein of 57 kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33, very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.

Purification: Protein G affinity chromatography

Immunogen: Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the HPV-16 L1 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the HPV-16 L1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunoprecipitation: 0.5-1 μg/500 μg protein lysate,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the HPV-16 L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Human Papilloma Virus 16 Capsid Protein (HPV-16 L1)

Alternative Name: HPV-16 L1

Target Type: Viral Protein

Background: Reacts with a protein of 57  kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33, very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.