WT1 antibody

Catalog No. ABIN3025552

The Mouse Monoclonal anti-WT1 antibody (Clone WT1-857) (ABIN3025552) specifically detects WT1 in IHC (p). The antibody is reactive with Human and Rat samples.

Quick Overview for WT1 antibody (ABIN3025552)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: WT1-857

Product Details anti-WT1 Antibody

Purpose: WT1 Antibody

Purification: Protein G affinity chromatography

Immunogen: A recombinant human protein was used as the immunogen for the WT1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the WT1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
2. View the recombinant version of the < a href=../tds/recombinant-wt1-antibody-rwt1-857-v3411>WT1/857 mAb.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the WT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

UniProt: P19544

Pathways: Tube Formation