CD5 antibody (AA 450-495)

Catalog No. ABIN3025590

The Mouse Monoclonal anti-CD5 antibody is suitable to detect CD5 in samples from Human and Rat. It has been validated for IHC (p).

Quick Overview for CD5 antibody (AA 450-495) (ABIN3025590)

Target: CD5

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: CD5-54-F6

Product Details anti-CD5 Antibody

Binding Specificity: AA 450-495

Purpose: CD5 Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: A portion of amino acids 450-495 from the human protein was used as the immunogen for this antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the CD5 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

UniProt: P06127