HCAM antibody

Catalog No. ABIN3025614

The Mouse Monoclonal anti-HCAM antibody is suitable to detect HCAM in samples from Human and Mouse. It has been validated for IHC (p).

Quick Overview for HCAM antibody (ABIN3025614)

Target: HCAM

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HCAM-1097

Product Details

Purpose: HCAM Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the HCAM antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the HCAM antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the HCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: HCAM

Background: Recognizes a cell surface glycoprotein of 80-95 kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44/HCAM immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

UniProt: P16070