TYR antibody

Catalog No. ABIN3025624

The Mouse Monoclonal anti-TYR antibody (Clone T311-OCA1-812) (ABIN3025624) specifically detects TYR in IHC (p). The antibody is reactive with Human samples.

Quick Overview for TYR antibody (ABIN3025624)

Target: TYR (Tyrosinase (TYR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYR antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: T311-OCA1-812

Product Details anti-TYR Antibody

Purpose: TYR Antibody Cocktail (Tyrosinase)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the TYR antibody cocktail.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the TYR antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the TYR antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for TYR

Target: TYR (Tyrosinase (TYR))

Alternative Name: TYR

Background: Recognizes a cluster of proteins between 70-80 kDa, identified as Tyrosinase/TYR. Occasionally a minor band at 55 kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.

UniProt: P14679