Nucleolin antibody

Catalog No. ABIN3025707

The Mouse Monoclonal anti-Nucleolin antibody has been validated for WB, IF, FACS and IHC (p). It is suitable to detect Nucleolin in samples from Human, Mouse, Rat, Cow, Pig, Rabbit, Guinea Pig, Chicken, Cat and Gerbil.

Quick Overview for Nucleolin antibody (ABIN3025707)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Guinea Pig, Chicken, Cat, Gerbil

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NCL-902

Product Details anti-Nucleolin Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the Nucleolin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,IF: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Western blot: 0.5-1 μg/mL,Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Recognizes a protein of ~76  kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Pathways: Ribonucleoprotein Complex Subunit Organization