VCAM1 antibody

Catalog No. ABIN3025722

The Mouse Monoclonal anti-VCAM1 antibody has been validated for FACS, IF and BP. It is suitable to detect VCAM1 in samples from Human.

Quick Overview for VCAM1 antibody (ABIN3025722)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Blocking Peptide (BP)

Clone: B-K9

Product Details anti-VCAM1 Antibody

Purification: Protein G affinity chromatography

Immunogen: Activated human umbilical vein endothelial cells (HUVEC) were used as the immunogen for the VCAM1 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the VCAM1 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL, Functional blocking: order BSA/azide free format

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the VCAM1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: VCAM1

Background: Recognizes a protein of 110  kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1), L1CAM and INCAM-100). It is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. L1CAM serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This mAb inhibits the binding of leukocytes to L1CAM on stimulated endothelial cells.

Pathways: Carbohydrate Homeostasis