beta-2 Microglobulin antibody

Catalog No. ABIN3025749

The Mouse Monoclonal anti-beta-2 Microglobulin antibody has been validated for FACS, IF, IP and IHC (fro). It is suitable to detect beta-2 Microglobulin in samples from Human and Non-Human Primate.

Quick Overview for beta-2 Microglobulin antibody (ABIN3025749)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BBM-1

Product Details anti-beta-2 Microglobulin Antibody

Purification: Protein G affinity chromatography

Immunogen: MOLT-4 human T cell line was used as the immunogen for the Beta-2 Microglobulin antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunoprecipitation: 2 μg/mg of lysate,Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Beta-2 Microglobulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: beta-2 Microglobulin

Background: Recognizes a protein of 12  kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an alpha- heavy chain that contains three subdomains (alpha-1, alpha-2, alpha-3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha-3 subdomain of the alpha- heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The alpha-1 and alpha-2 domains of the alpha- heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process