Liver Arginase antibody (AA 1-150)

Catalog No. ABIN3025825

The Mouse Monoclonal anti-Liver Arginase antibody (Clone ARG1-1125-ARG1-1126) (ABIN3025825) specifically detects Liver Arginase in IHC (p). The antibody is reactive with Human samples.

Quick Overview for Liver Arginase antibody (AA 1-150) (ABIN3025825)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ARG1-1125-ARG1-1126

Product Details anti-Liver Arginase Antibody

Binding Specificity: AA 1-150

Purpose: Arginase 1 Antibody Cocktail

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody cocktail.

Isotype: IgG3, kappa

Application Details

Application Notes: Optimal dilution of the Arginase 1 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Arginase 1 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: Arginase 1

Background: Recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes, which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin