Fascin antibody

Catalog No. ABIN3025904

This Mouse Monoclonal antibody specifically detects Fascin in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Fascin antibody (ABIN3025904)

Target: Fascin (FSCN1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FSCN1-417

Product Details anti-Fascin Antibody

Purification: Protein G affinity chromatography

Immunogen: Full length recombinant human protein was used as the immunogen for the Fascin antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the Fascin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: Fascin

Background: Recognizes a protein of 55  kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.