CD36 antibody

Catalog No. ABIN3025961

This Mouse Monoclonal antibody specifically detects CD36 in FACS, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD36 antibody (ABIN3025961)

Target: CD36

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD36 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GPIIIb-654

Product Details anti-CD36 Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the CD36 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the CD36 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Immunohistology (Formalin-fixed: 0.5-1 μg/mL for 30 minutes at RT (1),Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD36 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD36

Target: CD36

Alternative Name: CD36

Background: Recognizes a protein of 80  kDa-90  kDa, identified as CD36. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4 % of Japanese and East Asia population lack CD36.

Pathways: TLR Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins