GRP94 antibody

Catalog No. ABIN3025969

The Rat Monoclonal anti-GRP94 antibody (Clone 9G10-F8-2) (ABIN3025969) specifically detects GRP94 in WB, FACS, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GRP94 antibody (ABIN3025969)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: 9G10-F8-2

Product Details anti-GRP94 Antibody

Purpose: GRP94 Antibody / HSP90B1 (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the GRP94 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the GRP94 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: GRP94

Background: Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades