MYH11 antibody

Catalog No. ABIN3026118

The Mouse Monoclonal anti-MYH11 antibody has been validated for IF, FACS and IHC (p). It is suitable to detect MYH11 in samples from Human, Rat, Cat, Chicken, Cow, Dog, Guinea Pig, Pig and Rabbit.

Quick Overview for MYH11 antibody (ABIN3026118)

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Reactivity: Human, Rat, Cat, Chicken, Cow, Dog, Guinea Pig, Pig, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYH11 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ID8

Product Details anti-MYH11 Antibody

Purification: Protein G affinity chromatography

Immunogen: Human uterus extract was used as the immunogen for the SM-MHC antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the SM-MHC antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the SM-MHC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MYH11

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Alternative Name: SM-MHC

Background: SM-MHC, or Myosin-11, is a smooth muscle myosin belonging to the myosin heavy chain family. Myosin-11 is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It is a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. [Wiki]