MYH11 antibody

Catalog No. ABIN6940156

The Mouse Monoclonal anti-MYH11 antibody (Clone MYH11-923) (ABIN6940156) specifically detects MYH11 in IHC, IF and FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for MYH11 antibody (ABIN6940156)

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYH11 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MYH11-923

Product Details anti-MYH11 Antibody

Purpose: Smooth Muscle Myosin Heavy Chain (SM-MHC) Antibody

Specificity: Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

Cross-Reactivity (Details): Predicted to have broad species reactivity.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MYH11 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Uterus or normal breast.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MYH11

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Alternative Name: MYH11

Background:

AAT4, Myosin heavy chain 11 (MYH11), Myosin heavy chain 11 smooth muscle, Smooth muscle myosin heavy chain 11, Myosin-11, SM1, SM2, SMHC, SMMHC

Uterus or normal breast.

Molecular Weight: 205kDa (MHC-1) and 200kDa (MHC-2)

Gene ID: 4629, 460109

UniProt: P35749