Myeloid Cells antibody

Catalog No. ABIN3026143

The Mouse Monoclonal anti-Myeloid Cells antibody (Clone SPM298) (ABIN3026143) specifically detects Myeloid Cells in FACS, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Myeloid Cells antibody (ABIN3026143)

Target: Myeloid Cells

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM298

Product Details

Purification: Protein G affinity chromatography

Immunogen: Human PBMs were used as the immunogen for this Myeloid Cell antibody.

Isotype: IgG1

Application Details

Application Notes: The optimal dilution of the Myeloid Cell antibody for each application should be determined by the researcher.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Myeloid Cell antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Myeloid Cells

Alternative Name: Myeloid Cell

Background: SPM298 Ab reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation.