Melanoma Cell Marker antibody

Catalog No. ABIN3026335

The Mouse Monoclonal anti-Melanoma Cell Marker antibody (Clone M2-7C10-M2-9E3-HMB45-T311) (ABIN3026335) specifically detects Melanoma Cell Marker in IHC (p). The antibody is reactive with Human samples.

Quick Overview for Melanoma Cell Marker antibody (ABIN3026335)

Target: Melanoma Cell Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: M2-7C10-M2-9E3-HMB45-T311

Product Details

Characteristics: This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Purification: Protein G affinity chromatography

Immunogen: Recombinant MART-1 protein (M2-7C10 & M2-9E3), recombinant tyrosinase protein (T311) and extract of pigmented melanoma metastases from lymph nodes (HMB45) were used as the immunogens for this Melanoma Cell Marker antibody cocktail.

Isotype: IgG1, IgG2a, IgG2b

Application Details

Application Notes: The optimal dilution of the Melanoma Cell Marker antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Melanoma Cell Marker antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Melanoma Cell Marker

Background: This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.