GNAS antibody (AA 385-394)

Catalog No. ABIN302636

The Rabbit Polyclonal anti-GNAS antibody is suitable to detect GNAS in samples from Human, Monkey and Primate. It has been validated for IHC (p) and EIA.

Quick Overview for GNAS antibody (AA 385-394) (ABIN302636)

Target: GNAS (GNAS Complex Locus (GNAS))

Reactivity: Human, Monkey, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAS antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GNAS Antibody

Binding Specificity: AA 385-394

Specificity: This antibody detects GNAS at amino acids 385 to 394.

Purification: Protein G column

Immunogen: Synthetic peptide corresponding to amino acids 385 to 394 of human GNAS - KLH conjugated

Application Details

Application Notes: Immunohistochemistry on paraffin sections (20 μg/mL). ELISA (1: 0 - 1: 1000). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Alternative Name: GNAS1 / XLalphas

Background: The GNAS1 gene encodes the alpha subunit of the G protein Gs, which couples receptor binding by several hormones to activation of adenylate cyclase (Hayward et al., 1998). This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript exists, and this antisense transcript and one of the transcripts are paternally expressed, produce noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.Synonyms: Adenylate cyclase-stimulating G alpha protein, Extra large alphas protein, G protein alpha XLas, Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas

Gene ID: 2778

NCBI Accession: NP_536350

UniProt: Q5JWF2

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis