HSPD1 antibody

Catalog No. ABIN3026478

The Mouse Monoclonal anti-HSPD1 antibody has been validated for WB, IF and FACS. It is suitable to detect HSPD1 in samples from Human, Mouse, Rat, Chicken, Monkey, Hamster, Guinea Pig, Saccharomyces cerevisiae, E. coli, Nematode, Spinach, Trichinella spiralis, Salmonella typhimurium, Bacteria (Treponema), Borrelia, Streptococcus pyogenes and Yersinia enterocolitica.

Quick Overview for HSPD1 antibody (ABIN3026478)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Hamster, Guinea Pig, Saccharomyces cerevisiae, E. coli, Nematode, Spinach, Trichinella spiralis, Salmonella typhimurium, Bacteria (Treponema), Borrelia, Streptococcus pyogenes, Yersinia enterocolitica

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: LK2

Product Details anti-HSPD1 Antibody

Characteristics: This antibody recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). The antibody's epitope is localized between amino acids 383-419 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog. Clone LK2 antibody, unlike LK1 antibody, recognizes both mammalian and bacterial HSP60.

Purification: Protein G affinity chromatography

Immunogen: Recombinant human HSP60 protein was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSP60

Background: This antibody recognizes a 60  kDa protein, identified as the heat shock protein 60 (hsp60). The antibody's epitope is localized between amino acids 383-419 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog. Clone LK2 antibody, unlike LK1 antibody, recognizes both mammalian and bacterial HSP60.

Gene ID: 3329

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity