CD5 antibody

Catalog No. ABIN3026491

The Mouse Monoclonal anti-CD5 antibody (Clone CRIS-1) (ABIN3026491) specifically detects CD5 in FACS, IF, IP and Func. The antibody is reactive with Human samples.

Quick Overview for CD5 antibody (ABIN3026491)

Target: CD5

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func)

Grade: Carrier-free

Clone: CRIS-1

Product Details anti-CD5 Antibody

Purpose: CD5 Antibody (azide and preservative free)

Characteristics: This antibody recognizes a 67 kDa transmembrane protein, which is identified as CD5. It is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in Tcell areas. CD5 antibody is a pan Tcell marker that also reacts with a range of neoplastic Bcells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large Bcell lymphoma. Aberrant expression is useful in making a diagnosis of mature Tcell neoplasms. CD5 antibody detection is diagnostic in CLL/SLL within a panel of other Bcell markers, especially one that includes anti-CD23. CD5 antibody is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, it can be used in distinguishing thymic carcinoma (+) from thymoma (-). It does not react with granulocytes or monocytes.

Purification: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as the immunogen for this CD5 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD5 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: This antibody recognizes a 67 kDa transmembrane protein, which is identified as CD5. It is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in Tcell areas. CD5 antibody is a pan Tcell marker that also reacts with a range of neoplastic Bcells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large Bcell lymphoma. Aberrant expression is useful in making a diagnosis of mature Tcell neoplasms. CD5 antibody detection is diagnostic in CLL/SLL within a panel of other Bcell markers, especially one that includes anti-CD23. CD5 antibody is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, it can be used in distinguishing thymic carcinoma (+) from thymoma (-). It does not react with granulocytes or monocytes.