CD68 antibody

Catalog No. ABIN3026529

The Mouse Monoclonal anti-CD68 antibody is suitable to detect CD68 in samples from Human. It has been validated for IHC (p).

Quick Overview for CD68 antibody (ABIN3026529)

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: SPM130

Product Details anti-CD68 Antibody

Purpose: Anti-CD68 Antibody (azide and preservative free)

No Cross-Reactivity: Pig (Porcine), Dog (Canine), Chicken

Characteristics: This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Purification: Protein G affinity chromatography

Immunogen: A human alveolar macrophage subcellular fraction was used as the immunogen for this anti-CD68 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the anti-CD68 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68

Background: This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

UniProt: P34810