BCL2L1 antibody (AA 3-14)

Catalog No. ABIN3026614

The Mouse Monoclonal anti-BCL2L1 antibody (Clone BX006) (ABIN3026614) specifically detects BCL2L1 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BCL2L1 antibody (AA 3-14) (ABIN3026614)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL2L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: BX006

Product Details anti-BCL2L1 Antibody

Binding Specificity: AA 3-14

Purpose: Bcl-X Antibody (azide and preservative free)

Sequence: QSNRELVVDF LS

Characteristics: This antibody recognizes a protein of 27 kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

Purification: Protein G affinity chromatography

Immunogen: Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2).

Isotype: IgG2a, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-X antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. This antibody reacts with both BclXS and BclXL.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Bcl-X antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: Bcl-X

Background: This antibody recognizes a protein of 27 kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling