Pan Muscle Actin (pan) antibody

Catalog No. ABIN3026683

The Mouse Monoclonal anti-Pan Muscle Actin antibody (Clone MSA-953) (ABIN3026683) specifically detects Pan Muscle Actin in IHC (p) and WB. The antibody is reactive with Human and Rat samples.

Quick Overview for Pan Muscle Actin (pan) antibody (ABIN3026683)

Target: Pan Muscle Actin

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Grade: Carrier-free

Clone: MSA-953

Product Details

Binding Specificity: pan

Purpose: Pan Muscle Actin Antibody (azide and preservative free)

Characteristics: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Purification: Protein G affinity chromatography

Immunogen: An amino acid sequence common among human skeletal, cardiac and smooth muscle actins was used as the immunogen for the pan Muscle Actin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the pan Muscle Actin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the pan Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: Pan Muscle Actin

Background: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

UniProt: P62736