TAG-72 antibody

Catalog No. ABIN3026694

The Mouse Monoclonal anti-TAG-72 antibody is suitable to detect TAG-72 in samples from Human. It has been validated for FACS, IF and IHC (p).

Quick Overview for TAG-72 antibody (ABIN3026694)

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TAG-72 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TAGP-4

Product Details anti-TAG-72 Antibody

Characteristics: This antibody recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 antibody with a GCDFP-15 antibody is valuable in the diagnosis of apocrine carcinoma.

Purification: Protein G affinity chromatography

Immunogen: Recombinant human TAG-72 protein was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.

1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the TAG-72 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for TAG-72

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Alternative Name: TAG-72

Background: This antibody recognizes an oncofetal antigen of 220  kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 antibody with a GCDFP-15 antibody is valuable in the diagnosis of apocrine carcinoma.

Gene ID: 182875