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MUC5AC antibody

MUC5AC Reactivity: Human, Mouse, Rat, Monkey, Cat, Rabbit, Chicken, Pig, Hedgehog IF, FACS, IHC (p) Host: Mouse Monoclonal 45M1 unconjugated
Catalog No. ABIN3026776
  • Target See all MUC5AC Antibodies
    MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))
    Reactivity
    • 88
    • 53
    • 38
    • 18
    • 17
    • 16
    • 14
    • 13
    • 12
    • 6
    • 1
    • 1
    Human, Mouse, Rat, Monkey, Cat, Rabbit, Chicken, Pig, Hedgehog
    Host
    • 58
    • 57
    • 1
    Mouse
    Clonality
    • 59
    • 57
    Monoclonal
    Conjugate
    • 60
    • 16
    • 10
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MUC5AC antibody is un-conjugated
    Application
    • 54
    • 41
    • 31
    • 29
    • 28
    • 19
    • 18
    • 17
    • 15
    • 13
    • 13
    • 12
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    No Cross-Reactivity
    Cow (Bovine)
    Characteristics
    This antibody recognizes the peptide core of gastric mucin M1 (>1,000 kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
    Purification
    Protein G affinity chromatography
    Immunogen
    M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.
    Clone
    45M1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product MUC5AC Primary Antibody
  • Application Notes
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the MUC5AC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))
    Alternative Name
    MUC5AC Mucin-5AC (MUC5AC Products)
    Synonyms
    MUC5 antibody, TBM antibody, leB antibody, 2210005L13Rik antibody, MGM antibody, mucin 5AC, oligomeric mucus/gel-forming antibody, putative gastric mucin antibody, mucin 5, subtypes A and C, tracheobronchial/gastric antibody, mucin 2, oligomeric mucus/gel-forming antibody, MUC5AC antibody, Smp_144070 antibody, Muc5ac antibody, MUC2 antibody
    Background
    This antibody recognizes the peptide core of gastric mucin M1 (>1,000  kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
    Gene ID
    4586
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