Nuclear Marker antibody

Catalog No. ABIN3026799

The Mouse Monoclonal anti-Nuclear Marker antibody is suitable to detect Nuclear Marker in samples from Human, Rat and Mouse. It has been validated for IF and IHC (p).

Quick Overview for Nuclear Marker antibody (ABIN3026799)

Target: Nuclear Marker

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nuclear Marker antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: NM106

Product Details anti-Nuclear Marker Antibody

Purpose: Nuclear Marker Antibody (azide and preservative free)

No Cross-Reactivity: Pig (Porcine)

Characteristics: This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.

Purification: Protein G affinity chromatography

Immunogen: Nuclei of HL60 cells were used as the immunogen for the Nuclear Marker antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Nuclear Marker antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Nuclear Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nuclear Marker

Target: Nuclear Marker

Background: This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.