Fc gamma RII (CD32) antibody

Catalog No. ABIN3026914

The Mouse Monoclonal anti-Fc gamma RII antibody (Clone 7-3) (ABIN3026914) specifically detects Fc gamma RII in IF and Func. The antibody is reactive with Human samples.

Quick Overview for Fc gamma RII (CD32) antibody (ABIN3026914)

Target: Fc gamma RII (CD32)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Functional Studies (Func)

Grade: Carrier-free

Clone: 7-3

Product Details

Purpose: CD32 Antibody / FCGR2A (azide and preservative free)

Characteristics: This mAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

Purification: Protein G affinity chromatography

Immunogen: K562 and FcgRII+L cells were used as the immunogen for the CD32 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the CD32 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32

Background: This mAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

UniProt: P12318