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Secretory Component Glycoprotein antibody

Reactivity: Human, Rat IHC (p), IF, FACS Host: Mouse Monoclonal SPM217 unconjugated
Catalog No. ABIN3026937
  • Target See all Secretory Component Glycoprotein products
    Secretory Component Glycoprotein
    Reactivity
    Human, Rat
    Host
    • 3
    Mouse
    Clonality
    • 3
    Monoclonal
    Conjugate
    • 3
    This Secretory Component Glycoprotein antibody is un-conjugated
    Application
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    Characteristics
    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
    Purification
    Protein G affinity chromatography
    Immunogen
    Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.
    Clone
    SPM217
    Isotype
    IgG1 kappa
  • Application Notes
    The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Secretory Component antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Secretory Component Glycoprotein
    Abstract
    Secretory Component Glycoprotein Products
    Background
    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
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