Topoisomerase II alpha antibody

Catalog No. ABIN3027014

This Mouse Monoclonal antibody specifically detects Topoisomerase II alpha in WB, IF, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Topoisomerase II alpha antibody (ABIN3027014)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TPM2A-2

Product Details anti-Topoisomerase II alpha Antibody

Characteristics: This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Purification: Protein G affinity chromatography

Immunogen: Human partial recombinant protein was used as the immunogen for this Topoisomerase II alpha antibody.

Isotype: IgG

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Topoisomerase II alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topoisomerase II alpha

Background: This mAb recognizes a 170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Gene ID: 7153

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases